pGLO Analysis Questions

1.

2.  Two new traits that our transformed bacteria had was Ampicilin and arabinose sugar

3. For the -pGLO LB and -pGLO LB/amp , there are no colonies because they did not get the plasmid.

For the +pGLO LB/amp, there are about 5 colonies because it got the plasmid. For the +pGLO LB/amp/ara, there are about 30 colonies because it has arabinose sugar which leads to getting more colonies and plasmids

4. To allow pGLO to grow more colonies of bacteria

5. GFP is used for tagging genes for clearing expression or limiting profiles, studying protein to protein interactions and visualizing promoter activity
6. An example of genetic engineering is cloning.