Candy Electrophoresis Lab

1. The dyes are dyes that match the reference ones. Our dye bands were the same size, the colors were the same than the reference bands. It had only one color band and the dyes moved in the right direction. 

2. Fast Green FCF, because it has more negative charge and is also a lighter color.

3. Manufacturers put food coloring in dog food to attract people who have a dog buy it.

4. FD&C Dye       Color                Natural Alternative                             Source of Natural Dye

    Red 40               Red         Anthocyanins, Carmine, Cochineal                Bugs, Candy

    Yellow 5           Yellow       Turmeric, Beta Carotine, Annatto            Chips, jams, Candy

    Blue 1                Blue       Grape juice concentrate, Grape skin extract  Indigo, Woad Plant

    Red 40 Lake      Red      Grape juice concentrate, Grape skin extract  Bugs, Aluminum Hydroxide,                                                                                                                      Candy

    Blue 2 Lake       Blue        Coal Tar, used to color denim jeans         Makeup Products, Pill Coatings

    Yellow 6 Lake   Yellow     Paprika, Beta Carotine, Annatto              Foods, Drugs, Cosmetics

5. The size of the Molecule and the electrical charge

6. Gel Electrophoresis

7. They are separated when the DNA is put into the wells of the Gel box. This happens when the power goes on. When it goes on, the molecules, which have a negative charge move towards the positive electrode because opposite charges attract. 

8. The molecules with less Daltons separate quickly with 600 Daltons. It will move deep in the Gel With 1000 Daltons, molecules will spread quickly, but will spread slower than 600 Daltons. It will move deep in the gel, but not as deep as 600 Daltons. With 2000 Daltons, It will spread slower than 1000 Daltons, but it will not move as deep as 1000 Daltons. With 5000 Daltons, It will spread very slow and will not move very deep in the gel.